Akụkọ

A ga-ebu ụzọ tinye akwa iko ọhụrụ ma ọ bụ ihe eji eme ihe na mmiri iji mee ka ọ dị nro ma gbazee ihe ndị ahụ. Ekwesịrị iji mmiri mgbata saa ihe ọhụrụ enyo tupu ejiri ya, wee tinye ya na 5% hydrochloric acid n'abali; A na-ejikọta iko iko eji eme ihe na ọnụ ọgụgụ buru ibu nke protein na griiz, akọrọ mgbe ọ dịghị mfe ịchacha ya, n'ihi ya, a ga-etinye ya ozugbo na mmiri dị ọcha maka ịchacha.

1. Ihe ndị chọrọ nlebara anya:

Mgbe ihicha na disinfection tupu ejiri ya, ma efere petri dị ọcha ma ọ bụ na ọ naghị enwe mmetụta dị ukwuu na ọrụ ahụ, nwere ike imetụta ph nke omenala omenala, ma ọ bụrụ na e nwere ụfọdụ chemicals, ga-egbochi uto nke nje bacteria.

Ekwesịrị iji mmiri ọkụ sachaa efere ọhụrụ petri ọhụrụ ahụ, wee mikpuo ya na ngwọta hydrochloric acid nke nwere akụkụ dị nta nke 1% ma ọ bụ 2% ruo ọtụtụ awa iji wepụ ihe ndị na-adịghị na alkaline, wee kpochaa ya na mmiri distilled ugboro abụọ.

Ọ bụrụ na ị chọrọ omenala bacteria, mgbe ahụ, na-eji elu mgbali uzuoku (n'ozuzu 6.8 * 10 5 Pa elu mgbali uzuoku), sterilization na 120 ℃ maka 30min, akọrọ na ụlọ okpomọkụ, ma ọ bụ akọrọ okpomọkụ igba ogwu, bụ itinye petri efere na oven. , okpomọkụ akara na banyere 120 ℃ n'okpuru ọnọdụ nke 2h, ị nwere ike igbu nje ezé.

Enwere ike iji efere petri gbasiri ike naanị maka inoculation na omenala.

2. Jiri usoro:

Debe karama reagent nke a ga-eji mee ihe n'ọnọdụ kwesịrị ekwesị na mpaghara ọrụ, ma hapụ okpu nke karama reagent a ga-eji.

Debe efere petri n'etiti ebe ọrụ gị;

Wepu okpu nke karama reagent ma were pipette siphon reagent na karama reagent.

Tinye ihe mkpuchi nke efere petri n'azụ ya;

Jiri nwayọọ gbanye omenala omenala ozugbo n'ime isi nke otu akụkụ nke efere;

Tinye mkpuchi na efere petri;

Debe efere ahụ n'akụkụ ya, kpachara anya ka ị ghara ikwe ka onye na-ajụ ase banye n'ime obere oghere n'etiti mkpuchi na ala;

Wepu ahịhịa eji eme ihe.